Coding

Part:BBa_K4223019:Design

Designed by: RongKai Tang   Group: iGEM22_HainanU_China   (2022-09-29)


Cas13a protein-coding gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1063
    Illegal PstI site found at 1918
    Illegal PstI site found at 2857
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1063
    Illegal PstI site found at 1918
    Illegal PstI site found at 2857
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 344
    Illegal BglII site found at 1280
    Illegal BglII site found at 1490
    Illegal BglII site found at 1544
    Illegal BglII site found at 1775
    Illegal BglII site found at 2048
    Illegal BglII site found at 2534
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1063
    Illegal PstI site found at 1918
    Illegal PstI site found at 2857
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1063
    Illegal PstI site found at 1918
    Illegal PstI site found at 2857
    Illegal NgoMIV site found at 1996
    Illegal NgoMIV site found at 2632
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1


Source

1

References